A Multi-cell, Multi-scale Model of Vertebrate Segmentation and Somite Formation

Somitogenesis, an early developmental process common to all vertebrates, is an ideal problem for the application of multi-scale, cell-based computational modelling approaches. By combining current submodels of somitogenesis mechanisms at different scales, our 2D model was able to reproduce many features of this important developmental process.

On the left is shown the addition of new PSM cells (green) at the posterior end of the embryo. Cell colors represent the gradual accumulation of adhesion molecules: red cells express increasing level or EphA4; blue cells express increasing levels of ephrinB2; and dark green cells express increasing levels of N-Cadherin.

Center: Gradient of Fgf8 protein being produced by the PSM cells.

Right: Intracellular expression of lunatic fringe (Lfng), a key component of the somitic clock.

(a high resolution video is available: SomitesHighRes.wmv)

The CompuCell3D Model is available here: BelmonteHesterSomite.zip

For more details see: Hester SD, Belmonte JM, Gens JS, Clendenon SG, Glazier JA (2011) A Multi-cell, Multi-scale Model of Vertebrate Segmentation and Somite Formation. PLoS Comput Biol 7(10): e1002155